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Pstream fragment while PacI and BfrBI sites were introduced into the downstream fragments. The individual sequences were digested and ligated into the similarly digested temperature sensitive replication vector pML563 (Song et al., 2008) flanking the loxP-hyg-loxP cassette, and the resulting plasmid was named pML564. The cloned fragments were sequenced to ensure the absence of PCR errors. The pla
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But after that, we have to have fusion power. " In France, the International Thermonuclear Experimental Reactor (ITER) is the world's largest science experiment, and aims to prove that fusion can be achieved on a mass scale.
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Continuous hydrophobic outer surface as observed in all outer membrane proteins including MspA. In addition, the height of this OmpATb heptamer was 3 nm, which may be sufficient to span a planar lipid membrane in vitro, but is too short to span the 8 nm thick mycobacterial outer membrane (Hoffmann et al., 2008). Taken together, a critical evaluation of all experiments strongly indicates that Omp
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