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(V3)-E2 construct was refolded with equimolar amounts of E2wt(V3)-E2 construct was refolded with equimolar amounts of E2wt, requiring a 1:1 ratio of Env-E2 fusion protein: E2wt to [19] remain fully soluble . Solubilized particles typically have more than 50 EU/mL of E. coli-derived endotoxin (lipopolysaccharide, LPS) as a result of expression in this system. Endotoxin levels can be reduced t

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